Nested Polymerase Chain Reaction for Detection of Hepatitis C Virus RNA in Blood Derivatives

Nested polymerase chain reaction for detection of hepatitis C virus RNA in blood derivatives.

Testing for the presence of hepatitis C virus (HCV) in blood derivatives used in clinical medicine is of enormous importance to ensure the production of safe preparations. So far, no system has been developed for the isolation and detection of hepatitis C virus from blood derivatives. The recently introduced commercial kit for the detection of hepatitis C virus is designed for the isolation and...

evaluation of multiplex nested polymerase chain reaction for routine hepatitis c virus genotyping in egyptian patients

Evaluation of Multiplex Nested Polymerase Chain Reaction for Routine Hepatitis C Virus Genotyping in Egyptian Patients

BACKGROUND At least six HCV (hepatitis C virus) genotypes are unequally distributed worldwide. HCV genotyping guides the selection of treatment regimens and provides important epidemiological markers that enable the outbreak source to be traced and the spread of disease to be controlled. In Egypt, there is an increasing need for cost-effective, fast, and easily performable HCV genotyping assays...

Detection of hepatitis C virus RNA by a combined reverse transcription-polymerase chain reaction assay.

Amplification of RNA by the polymerase chain reaction (PCR) is normally a two-step process requiring separate enzymes and buffer conditions. We describe a combined reverse transcription-PCR (RT-PCR) assay for hepatitis C virus (HCV) RNA amplification in which a single enzyme and buffer condition are used. In this assay, both the RT and PCR steps are carried out with the thermoactive DNA polymer...

Detection Of Toxoplasma Gondii and Human Cytomegalovirus DNA in Blood from Transplant Recipients Using Multiplex Nested Polymerase Chain Reaction

Evidences from many studies suggested a polymerase chain reaction (PCR) as a valuable method for diagnosing infectious disease in the transplant recipients. We used this method for detection of Toxoplasma, gondii and human cytomegalovirus in blood specimens from patients after bone marrow or kidney transplantation. DNA of both infectious agents were detected using two separate sets of nested pr...